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NHMFL







Timothy Cross

Dr. Timothy A. Cross

NMR Program Director, Professor of Chemistry
CIMAR, NMR

Email: 
Phone: 850 - 644-0917
Alt. Phone: 850 - 339-3186
Fax: 850 - 644-1366
Address: B232 NHMFL FSU
1800 E. Paul Dirac Dr.
Tallahassee, FL 32310-3706

Educational Background:
Trinity  College    Hartford, CT B.S. 1976 Chemistry
University of Penn.    Philadelphia, PA Ph.D. 1981 Chemistry
University of Penn. Philadelphia, PA Postdoc '81-'83 Chemistry
Universität Basel Basel, Switzerland Postdoc '83-'84 Biophys. Chem.

Research Interests:
- Understanding how biological macromolecules, specifically membrane proteins, carry out their function
- Structural Biology and Structural Genomics
- Structural and Dynamic characterization of membrane proteins
- Development of solid state NMR methodology for macromolecular studies.
- Development of high field NMR technology and applications

Professional Experience:
Department of Chemistry, Florida State University
Assistant & Associate Professor 1984-1993
Professor 1993-
Director of the FSU Nuclear Magnetic Resonance Facility 1984-
Department of Biological Science Courtesy Associate Professor 1997-
Institute of Molecular Biophysics, Florida State University 1984-
Director of the Institute & Structural Biology Program 1991-1993
Center for Materials Res. & Tech., Florida State University 1990-
National High Magnetic Field Lab., Florida State University 1990-
Acting Director  NMR Program 1991-1994
Director for New Initiatives 1995-1997
Director of the NMR Program 1998-

Awards, Honors & Service:
Fellow of the Biophysical Society (2007)
Earl Frieden Professorship, FSU (2003)
Distinguished Research Professor, Florida State University (2001)
Biophysical Society Council Member (2000-2002)
Editorial Board - Biophysical Journal (1998-2004)
Editorial Board - J. Magnetic Resonance (1998-2003)
Fellow of the American Association for the Advancement of Science (1998-present)
Advisory Board - Magnetic Resonance in Chemistry (1997-2000)
NIH BBCB Study Section (1995-1999)
NIH Shared Instrumentation Panel (1993)
NIH Special Study Section (1993)
Florida State University Developing Scholar Award (1990-1991)
NSF Biophysics Review Panel (1989-1992)
NSF ILI Review Panel (1989)
Alfred P. Sloan Research Fellow (1988-1990)
NIH Molecular Cytology Study Section (ad hoc) (1988)
NSF Presidential Young Investigator Award (1985-1990)
NATO Postdoctoral Fellowship (1983-1984)
NIH Predoctoral Trainee in Cell and Molecular Biology (1978-1981)
Honors in Chemistry and General Scholarship (1976)
Mary A. Terry Fellowship (1976-1978)
Phi Beta Kappa (1975-present)

Disclaimer: The information presented below was automatically generated from a database. In the rare instance of an exact name match, it is possible that a citation is inaccurately attributed. The listing should therefore be considered unofficial. To report a mismatched publication, contact IMsystems@magnet.fsu.edu

2009 Publications & Scientific Activities

    Peer-Reviewed Publications
  • Qian, C.; Fu, R.; Gor'kov, P.L.; Brey, W.W; Cross, T.A. and Gan, Z., 14N Polarization Inversion Spin Exchange at Magic Angle (PISEMA), J. Magn. Reson., 196, 96–99 (2009)
  • Yi, M.; Cross, T.A. and Zhou, H.-X., Conformational heterogeneity of the M2 proton channel and a structural model for channel activation., P. Natl. Acad. Sci. U.S.A., 106, 13311-13316 (2009) [read online]
    Presentations, Posters & Abstracts
  • Yi, M.; Cross, T.A. and Zhou, H.-X., Conformational heterogeneity of the M2 proton channel: a model for channel activation, Biophysical Society 53rd Annual Meeting, Boston, MA, Feburary (2009) [read online]

2008 Publications & Scientific Activities

    Peer-Reviewed Publications
  • Achuthan, S.; Asbury, T.; Hu, J.; Bertram, R.; Cross, T.A. and Quine, J.R., Continuity Conditions and Torsion Angles From ssNMR Orientational Restraints, J. Magn. Reson., 191, 24-30 (2008) [read online]
  • Gan, Z.; Kwak, H.T.; Bird, M.; Cross, T.; Gor'kov, P.; Brey, W.W. and Shetty, K., High-field NMR using resistive and hybrid magnets, J. Magn. Reson., 191, 135-140 (2008) [read online]
  • Hu, J.; Qin , H.; Sharma, M.; Cross, T.A. and Gao, F.P., Chemical cleavage of fusion proteins for high-level production of transmembrane peptides and protein domains containing conserved methionines, Biochim. Biophys. Acta, 1778, 1060-1066 (2008) [read online]
  • Li, C.; Yi, M.; Zhou, H.-X. and Cross, T.A., Solid-state NMR and MD simulations of the antiviral drug amantadine solubilized in DMPC bilayers., Biophysical J., 94, 1295 (2008) [read online]
  • Moffat, J.C.; Vijayvergiya, V.; Gao, P.F.; Cross, T.A.; Woodbury, D.J. and Busath, D.D., Direct Measurement of Proton Transport Through Influenza Virus A M2 Protein Reconstituted in Vesicles, Biophysical J., 94, 434-445 (2008) [read online]
  • Nguyen, H. and Cross, T.A., Backbone 1H, 15N, and 13C assignment of Rv1567c, an integral membrane protein from Mycobacterium tuberculosis, Biomol. NMR Assign., 2, 47-49 (2008) [read online]
  • Page, R.C.; Kim, S. and Cross, T.A., Transmembrane helix uniformity examined by spectral mapping of torsion angles, Structure, 16 (5), 787-797 (2008) [read online]
  • Qin, H.; Hu, J.; Hua, Y.; Challa, S.V.; Cross, T.A. and Gao, F.P., Construction of a Ligation Independent Cloning Based Platform for High Throughput Cloning and Expression Screening of membrane Proteins from Mycobacterium tuberculosis, BMC Biotechnology, 8, 51 (2008) [read online]
  • Witter, R.; Nozirov, F.; Sternberg, U.; Cross, T.A.; Ulrich, A.S. and Fu, R., Solid-state 19F-NMR reveals that Trp41 participates in the gating mechanism of the M2 proton channel of Influenza A virus, J. Am. Chem. Soc., 130, 918-924 (2008) [read online]
  • Yi, M.; Cross, T.A. and Zhou, H.-X., A secondary gate as a mechanism for inhibitionof the M2 proton channel by amantadine., J. Phys. Chem. B, 112, 7977-7979 (2008) [read online]
    Presentations, Posters & Abstracts
  • Cross, T.A.; Blue, A.; Brey, W.W.; Brueschweiler, R.; Fu, R.; Gan, Z.; Gor’kov, P.L.; Grant, S.C.; Logan, T.M.; Paravastu, A.K.; Schepkin, V.D.; Shetty, K.K. and Zhang, F., NMR User Facility at the National High Magnetic Field Laboratory in Tallahassee Florida USA, 49th Experimental Nuclear Magnetic Resonance Conference (ENC), Asilomar, CA, March 9-14 (2008)
  • Gor'kov, P.; Qian, C.Q.; Kitchen, J.A.; Sharma, M.; Cross, T.A. and Brey, W.W., Applications of Low-E Coils to High Field SS NMR of Proteins Aligned in Bicelles, 49th ENC, Asilomar Conf. Grounds, Pacific Grove, CA, March 9-14 (2008)
  • Jones, T.L.; Ekstrom, B.; Cross, T.A.; Fu, R. and Busath, D.D., Persistent Gramicidin a Channel Gating, Biophysical Society 52nd Annual Meeting, Long Beach, CA, February (2008)
  • Moore, J.; Chauhan, A.; Rajagopalan, M. and Cross, T.A., Inducing Order in ChiZ, a Natively Disordered Membrane Protein from Mycobacterium tuberculosis, Biophysical Society 52nd Annual Meeting, Long Beach, CA, February (2008)
  • Nguyen, H.B.; Qin, H.; Rajagopalan, M.; Gao, P. and Cross, T.A, Solution Structure of Integral Membrane Protein from Mycobacterium tuberculosis: Insight into Rv0008c - an FtsZ Inhibitor and Its Complex, Joint Meeting of the Biophysical Society 52nd Annual Meeting and 16th Int. Biophysics Congress (IUPAB), Long Beach, CA, February 2-6 (2008)
  • Page, R. and Cross, T.A., Diving into the Spectral Depths: Torsion Angle Mapping as a Probe for Transmembrane Helix Uniformity, Biophysical Society 52nd Annual Meeting, Long Beach, CA, February (2008)
  • Schepkin, V.D.; Brey, W.B.; Falconer, N.D.; Grant, S.C.; Gor'kov, P.L.; Shetty, K.K. and Cross, T.A., The First in vivo Mouse Proton and Sodium MR Imaging at 21 T, Oral Presentation at the Annual Meeting of the International Society for Magnetic Resonance in Medicine, Toronto, Canada, May 3-9 (2008); Published in Proc. Annual Meeting ISMRM-2008, 808 (2008) [read online]
  • Sharma, M.; Li, C.; Gao, P.; Qin, H. and Cross, T.A., Solution and Solid State NMR Studies of M2 Proton Channel of Influenza A Virus, Annual Biophysics Society Meeting, Long Beach, CA, February 2-6 (2008)
  • Truong, M.; Fu, R. and Cross, T.A., High Resolution Heteronuclear Correlation Spectroscopy in Solid State NMR of Aligned Samples, 49th Experimental Nuclear Magnetic Resonance Conf. (ENC), Asilomar, CA, March 9-14 (2008)
  • Yi, M.; Cross, T.A. and Zhou, H.X., Helix Kinking, Conformational Heterogeneity, and Blockage of the M2 Proton Channel: Roles of Water Molecules and Amantadine Binding, Biophysical Society 52nd Annual Meeting, Long Beach, CA, February (2008)

2007 Publications & Scientific Activities

    Peer-Reviewed Publications
  • Fu, R.; Truong, M.; Saager, R.J.; Cotten, M. and Cross, T.A., High-resolution heteronuclear correlation spectroscopy in solid state NMR of aligned samples, J. Magn. Reson., 188, 41-48 (2007) [read online]
  • Hu, J.; Asbury, T.; Achuthan, S.; Li, C.; Bertram, R.; Quine, J.R.; Fu, R. and Cross, T.A., Backbone Structure of the Amantadine-blocked Transmembrane Domain M2 Proton Channel from Influenza A Virus, Biophysical J., 92, 4335-4343 (2007) [read online]
  • Hu, J.; Fu, R. and Cross, T.A., The Chemical and Dynamical Influence of the Anti-Viral Drug Amantadine on the M2 Proton Channel Transmembrane Domain, Biophysical J., 93, 276-283 (2007) [read online]
  • Hu, J.; Qin , H.; Li, C.; Sharma, M.; Cross, T.A. and Gao, F.P., Structural Biology of Transmembrane Domains: Efficient Production and Characterization of Transmembrane Peptides by NMR, Protein Science, 16 (10), 2153-65 (2007) [read online]
  • Li, C.; Gao, P.; Qin, H.; Chase, R.; Gor'kov, P.L.; Brey, W. W. and Cross, T.A., Uniformly Aligned Full-Length Membrane Proteins in Liquid Crystalline Bilayers for Structural Characterization, J. Am. Chem. Soc., 129 (17), 5304-5305 (2007) [read online]
  • Li, C.; Qin, H.; Gao, F.P. and Cross, T.A., Solid-State NMR Characterization of Conformational Plasticity within the Transmembrane Domain of the Influenza A M2 Proton Channel, Biochim. Biophys. Acta - Biomembranes, 1768, 3162-3170 (2007) [read online]
  • Page, R.C.; Li, C.; Hu, J.; Gao, F.P. and Cross, T.A., Lipid bilayers: an essential environment for the understanding of membrane proteins, Magnet. Reson. Chem., 45, S2-S11 (2007) [read online]
    Presentations, Posters & Abstracts
  • Chase, R.A.; Li, C.; Gao, P.F. and Cross, T.A., Characterization of Membrane Proteins from M. tuberculosis Using Solution and Solid State NMR, Biophysical Society 51st Annual Meeting, Baltimore, MD, February (2007)
  • Fu, R.; Truong, M.; Saager, R.J.; Cotten, M. and Cross, T.A., High Resolution Heteronuclear Correlation Spectroscopy in Solid State NMR of Aligned Samples, 48th ENC Conf., Daytona Beach, FL, April 22-27 (2007)
  • Fu, R.; Truong, M.; Saager, R.J.; Vollmar, B.; Cross, T.A. and Cotten, M., High Resolution Solid-State 1H-15N HETCOR Spectroscopy for Structural Characterization of Multiply-Labeled Peptides in Aligned Bilayers, Int. Society of Magnetic Resonance (ISMAR), Taiwan, October 14-19 (2007)
  • Fu, R.; Truong, M.; Saager, R.J.; Vollmar, B.S.; Daza, E.L.; Ditto, J.J.; Gordon, E.D.; Taylor, M.M.; Cross, T.A.; and Cotten, M., Dipolar Encoded 1H-15N HETCOR Spectroscopy for Structural Characterization of Aligned Samples, 36th Southeastern Magnetic Resonance Conference (SEMRC), Tuscaloosa, AL, November 9-11 (2007)
  • Nguyen, H.B. and Cross, T.A., Initial Characterization of Rv0008c, an Integral Membrane Protein from Mycobacterium tuberculosis by Solution NMR Spectroscopy, Experimental Nuclear Magnetic Resonance Conf., Daytona Beach, FL, April 22-27 (2007)
  • Nguyen, H.B.; Qin, H.; Gao, P. and Cross, T.A., Solution Structure of Membrane Proteins from Mycobacterium tuberculosis: Insight into Rv0008c and its Complex, NIH National Graduate Student Research Festival, Bethesda, MD, October 11-12 (2007)
  • Nguyen, H.B.; Qin, H.; Gao, P. and Cross, T.A., Structure Characterization of Rv0008c, an Integral Membrane Protein from Mycobacterium tuberculosis and Rv008c/Rv0011c Membrane Protein Complex by Solution Magnetic Resonance Spectroscopy (NMR), The 21st Symposium of Protein Society, Boston, MA, July 21-25 (2007)
  • Nielson, F.; Harris, T.; Jones, T.; Rassmussen, S.T.; Gao, P.; Cross, T.A. and Busath, D.D., External His Mutations Affect Proton Conductance of Influenza M2 Channels, Biophysical Society 51st Annual Meeting, Baltimore, MD, February (2007)
  • Nozirov, F.; Witter, R.; Ulrich, A.S.; Cross, T.A. and Fu, R., 19F Solid-state NMR Studies of the Transmembrane Domain of the M2 protein, 48th ENC, Experimental Nuclear Magnetic Resonance Conf., Daytona Beach, FL, April 22-27 (2007)
  • Page, R.C. and Cross, T.A., Transmembrane Helix Structure in Two Distinct Membrane Mimetics: Applying Solution and Solid State NMR to Examine the Structure of KdpC, 48th Experimental Nuclear Magnetic Resonance Conf., Daytona Beach Hilton Conf. Center, Daytona Beach, FL, April 22-27 (2007)
  • Sharma, M.; Li, C.; Gao, P.; Qin, H. and Cross, T.A., Solution and Solid State NMR Studies of M2 Proton Channel of Influenza A Virus, ENC Conf., Daytona, FL, April (2007)
  • Witter, R.; Sternberg, U.; Klipfel, M.; Heinzmann, R.; Gor’kov, P.; Nozirov, F.; Li, C.; Fu, R.; Cross, T.A. and Ulrich A.S., Structure and Dynamics Investigations with Solid State 13C-, 15N- and 19F-NMR Chemical Shift Tensors, 48th ENC, Experimental Nuclear Magnetic Resonance Conf., Daytona Beach, FL, April 22-27 (2007)
  • Yi, M.; Li, C.; Cross, T.A. and Zhou, H.-X., Molecular Dynamics Study of Amantadine Inhibition of the M2 Ion Channel of Influenza A in Phospholipid Bilayers, Biophysical Society 51st Annual Meeting, Baltimore, MD, February (2007)

2006 Publications & Scientific Activities

    Peer-Reviewed Publications
  • Asbury, T.; Quine, J.R.; Achuthan, S.; Hu, J.; Chapman, M.S.; Cross, T. A. and Bertram, R., PIPATH: An optimized algorithm for generating alpha-helical structures from PISEMA data, J. Magn. Reson., 183, 87-95 (2006) [read online]
  • Chekmenev, E.Y.; Gor'kov, P.L.; Cross, T.A.; Alaouie, A.M. and Smirnov, A.I., Flow-Through Lipid Nanotube Arrays for Structure-Function Studies of Membrane Proteins by Solid-State NMR Spectroscopy, Biophysical J., 91 (8), 3076-3084 (2006) [read online]
  • Chekmenev, E.Y.; Waddell, K.W.; Hu, J.; Gan, Z.; Wittebort, R.J. and Cross, T.A., Ion Binding Study by 17O Solid-State NMR Spectroscopy in a Model Peptide Gly-Gly-Gly at 19.6 T, J. Am. Chem. Soc., 128, 9847-9855 (2006) [read online]
  • Gao, F.P. and Cross, T.A, Recent Developments in membrane Protein Structural Genomics, Genome Biology, 6, 244 (2006) [read online]
  • Gor'kov, P.L.; Chekmenev, E.Y.; Fu, R.; Hu, J.; Cross, T.A.; Cotten, M. and Brey, W.W., A large volume flat coil probe for oriented membrane proteins, J. Magn. Reson., 181, 9-20 (2006) [read online]
  • Hu, J.; Fu, R.; Nishimura, K.; Zhang, L.; Zou, H.X.; Busath, D.D.; Vijayvergiya, V. and Cross, T.A., Histidines, heart of the hydrogen ion channel from influenza A virus: Toward an understanding of conductance and proton selectivity, P. Natl. Acad. Sci. U.S.A., 103, 6865-6870 (2006) [read online]
  • Li, C.; Mo, Y.; Hu, J.; Chekmenev, E.Y.; Tian, C.; Gao, F.P.; Fu, R.; Gor'kov, P.L.; Brey, W.W. and Cross, T.A., Analysis of RF heating and sample stability in aligned static solid state NMR spectroscopy, J. Magn. Reson., 180, 51-57 (2006) [read online]
  • Page, R.C.; Moore, J.D.; Nguyen, H.B.; Sharma, M.; Chase, R.; Gao, F.P.; Mobley, C.K.; Sanders, C.R.; Ma, L.; Sönnichsen, F.D.; Lee, S.; Howell, S.C.; Opella, S.J., and Cross, T.A., Comprehensive evaluation of solution nuclear magnetic resonance spectroscopy sample preparation for helical integral membrane proteins, J. Structural and Functional Genomics, 7 (1), 51-64 (2006) [read online]
  • Quine, J.R.; Achuthan, S.; Asbury, T.; Bertram, R.; Chapman, M.S.; Hu, J. and Cross, T.A., Intensity and Mosaic Spread Analysis from PISEMA Tensors in Solid State NMR., J. Magn. Reson., 179, 190-198 (2006) [read online]
    Presentations, Posters & Abstracts
  • Busath, D.; Goit, J.; Rasmussen, S.; Kiriyama, J.; Vijayvergiya, V.; Gao, P.F. and Cross, T.A., Conductance Properties of Influenza A Virus M2 Variants in Planar Lipid Bilayers, Biophysical Society 50th Annual Meeting, Salt Lake City, UT, February 18-22 (2006)
  • Chase, R.A.; Moore, J.A.; Nguyen, H.; Page, R.; Sharma, M.; Qin, H.; Gao, F.P. and Cross, T.A., Characterizing Membrane Proteins from M. Tuberculosis, Calculating Residual Dipolar Couplings, ENC, Asilomar, CA, April 23-28 (2006)
  • Chekmenev, E.Y.; Cross, T.A.; Alaouie, A.M.; Venkatesan, U. and Smirnov, A.I., Gramicidin A Channels in Substrate-Supported Lipid Nanotube Bilayers, Biophysical Society 50th Annual Meeting, Salt Lake City, UT, February 18-22 (2006)
  • Chekmenev, E.Y.; Gor'kov, P.L.; Cross, T.A.; Alaouie, A.M. and Smirnov, A.I., Flow-through Lipid Nanotube Arrays for Macroscopic Alignment and Structure-function Studies of Membrane proteins by Solid-state NMR Spectroscopy, ENC Conf., Asilomar, CA, April 23 8 (2006)
  • Chekmenev, E.Y.; Miller, L.N.; Hu, J.; Mo, Y.; Gor'kov, P.L.; Brey, W.W.; Waddell, K.W.; Wittebort, R.J. and Cross, T.A., Towards 17O Solid State NMR Spectroscopy of Ion-selective Channels at Ultra-high Magnetic Fields, 47th ENC, Asilomar, CA, April 23-28 (2006)
  • Cross, T.A.; Fu, R.; Brey, W.W.; Shetty, K.; Gor'kov, P.L.; Grant, S.C.; Gan, Z.; Zhang, F.; Logan, T.M.; and Blue, A., NMR User Facility at the National High Magnetic Field Laboratory in Tallahassee FL, 47th Experimental Nuclear Magnetic Resonance Conf., Asilomar, CA, April 23-28 (2006); Published in Proc. 47th ENC, 47, EO60345 (2006)
  • Gao, F.P.; Qin, H. and Cross, T.A., High-throughput Production of Integral Membrane Proteins for Nuclear Magnetic Resonance-based Structural Genomics, Biophysical Society 50th Annual Meeting, Salt Lake City, UT, February 18-22 (2006)
  • Gao, F.P.; Sharma,M.; Qin, H. and Cross,T.A., Solution Structure of the cytoplasmic Domain of the Influenza M2 Protein, Biophysical Society 50th Annual Meeting, Salt Lake City, UT, February 18-22 (2006)
  • Gor'kov, P.L.; Chekmenev, E.Y.; Cotten, M.; Veglia, G.; Buffy, J.J.; Traaseth, N.; Cross, T.A. and Brey, W.W., Applications of Low-E Static Probes to High Frequency Membrane Protein NMR, 47th ENC, Asilomar, CA, April 23-28 (2006)
  • Gor’kov, P.L.; Chekmenev, E.Y.; Li, C.; Cotten, M.; Veglia, G.; Buffy, J.J.; Traaseth, N.; Long, J.R.; McNeill, S.; Witter, R.; Nozirov, F.; Fu, R.; Cross, T.A. and Brey, W.W., Low-E Probes for High Frequency Biological SS NMR, Rocky Mountain Conf. on Analytical Chemistry, Breckenridge, CO, July 24-28 (2006)
  • Hu, J.; Gao, F.P; Sharma, M.; Qin, H. and Cross, T.A., Expression, Purification and Preliminary NMR Studies of the Transmembrane Domain of CorA, a Magnesium Transporter from Mycobacterium Tuberculosis, ENC Conf., Asilomar, CA, April 23-28 (2006)
  • Li, C. and Cross, T.A., Two Molecular Mechanisms toward Influenza A Virus Resistant to Antiviral Drug: Amantadine - a Solid State NMR Study, ENC Conf., Asilomar, CA, April 23-28 (2006)
  • Nguyen, H.B.; Moore, J.D.; Page, R.C.; Chase, R.; Shamar, M.; Qin, H.; Gao, P.F. and Cross, T.A., Effect of Paramagnetic Relaxation on Integral Membrane Proteins from Mycobacterium tuberculosis by Solution NMR Spectroscopy, ENC Conf., Asilomar, CA, April 23-28 (2006)
  • Nguyen, H.B.; Qin, H.; Gao, P. and Cross, T.A., Solution NMR of alpha-helical membrane proteins Rv0008c and Rv1567c from Mycobacterium tuberculosis, XXIInd Int. Conf. on Magnetic Resonance in Biological Systems, Göttingen, Germany, August 20-25 (2006)
  • Nozirov, F.; Witter, R.; Ulrich, A.S.; Cross, T.A and Fu, R., 19F Solid-state NMR Studies of the Transmembrane Domain of the M2 protein, 47th ENC Conf., Asilomar, CA, April 23-28 (2006)
  • Page, R.C.; Li, C.; Chase, R.A.; Gao, F.P. and Cross, T.A., Alpha-Helical Membrane Protein Structure by Solution and Solid State NMR: Examining KdpC and Rv1861 from Mycobacterium tuberculosis, Int. Conf. Magnetic Resonance in Biological Systems, Göttingen, Germany, August 20-26 (2006)
  • Qin, H.; Page, R.; Moore, J.; Nguyen, H.; Chen, R.; Hu, H.; Cross, T.A. and Gao, F.P., Structural Genomics of Integral Membrane Proteins from Mycobacterium tuberculosis, 4th ISGO Int. Conf. on Structural Genomics, Yokohama, Japan/Beijing, China, October 19-21/October 22-26 (2006)
  • Vijayvergiya, V.; Madson, S.L.; Gao, P.F.; Cross, T.A. and Busath, M.D., Proton Conductance of Influenza A Virus M2 Mutants and M2 TMP in Planar Lipid Bilayers, Biophysical Society 50th Annual Meeting, Salt Lake City, UT, February 18-22 (2006)
  • Witter, R.; Sternberg, U.; Nozirov, F.; Gor'kov, P.L.; Schmitt, M.; Mock, W.; Leidich, S.; Fu, R.; Cross, T.A. and Ulrich, A.S., New Theoretical and Experimental Concepts of Solid State 19F-NMR for Biological Applications, 47th ENC Conf., Asilomar, CA, April 23-28 (2006)
  • Witter, R.; Sternberg, U.; Nozirov, F.; Li, C.; Fu, R.; Klipfel, M. and Cross, T.A., Structure refinement of M2-TDM, 22nd Int. Conf. on Magnetic Resonance in Biological Systems, Göttingen, Germany, August 20-25 (2006)
  • Witter, R.; Sternberg, U.; Nozirov, F.; Li, C.; Fu, R.; Klipfel, M.; Cross, T.A. and Ulrich, A.S., Structure refinement of M2-TMD, Int. Conf. on Magnetic Resonance in Biological Systems, Göttingen, Germany, August 20-25 (2006); Published in Book of Abstracts (0)
  • Yi, M.; Carbone, I.; Cross, T.A. and Zhou, H.-X., Calculation of pKa's for histidine at the center of the influenza A M2 proton channel, Biophysical Society 50th Annual Meeting, Salt Lake City, UT, February 18-22 (2006)

2005 Publications & Scientific Activities

    Peer-Reviewed Publications
  • Chekmenev, E.Y.; Hu, J.; Gor'kov, P.L.; Brey, W.W.; Cross, T.A.; Ruuge, A. and Smirnov, A.I., 15N and 31P solid-state NMR study of transmembrane domain alignment of M2 protein of influenza A virus in hydrated cylindrical lipid bilayers confined to anodic aluminum oxide na, J. Magn. Reson., 173 (2), 322-327 (2005) [read online]
  • Fu, R.; Brey, W.W.; Shetty, K.; Gor'kov, P.; Saha, S.; Long, J.R.; Grant, S.C.; Chekmenev, E.Y.; Hu, J.; Gan, Z.; Sharma, M.; Zhang, F.; Logan, T.M.; Bruschweiler, R.; Edison, A.; Blue, A.; Dixon, I.; Markiewicz, W.D. and Cross, T.A., Ultra-Wide Bore 900 MHz High-Resolution NMR at the National High Magnetic Field Laboratory, J. Magn. Reson., 177, 1-8 (2005) [read online]
  • Hu, J.; Chekmenev, E.Y.; Gan, Z.; Gor'kov, P.L.; Saha, S.; Brey, W.W. and Cross, T.A., Ion Solvation by Channel Carbonyls Characterized by 17O Solid State NMR at 21 T, J. Am. Chem. Soc., 127 (34), 11922 - 11923 (2005) [read online]
  • Korepanova, A.; Gao, F.P.; Hua, Y.; Qin, H.; Nakamoto, R.K. and Cross, T.A., Cloning and expression of multiple integral membrane proteins from Mycobacterium tuberculosis in Escherichia coli, Protein Science, 14 (1), 148 (2005) [read online]
  • Xiong, Y.; Chalmers, M.J.; Gao, F.P.; Cross, T.A. and Marshall, T.A., Identification of Mycobacterium tuberculosis H37Rv Integral Membrane Proteins by One-Dimensional Gel Electrophoresis and Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry, J. Proteome Res., 4, 855-861 (2005) [read online]
    Presentations, Posters & Abstracts
  • Achuthan, S.; Hu, J.; Asbury, T.; Bertram, R.; Cross, T.A. and Quine, J.R., Analysis of Orientational Restraints from Solid State NMR for Membrane Protein Structure Determination, Annual Biophysics Society Meeting, Long Beach, CA, February (2005)
  • Chekmenev, E.Y.; Gor'kov, P.L.; Cross, T.A.; Alaouie, A.M. and Smirnov, A.I., Flow-through Lipid Nanotube Arrays for Macroscopic Alignment and Structure-function Studies of Membrane proteins by Solid-state NMR Spectroscopy, Providence, RI, ENC Conf., April 10-15 (2005)
  • Chekmenev, E.Y.; Hu, J.; Gan, Z.; Gor'kov, P.L.; Saha, S.; Brey, W.W. and Cross, T.A., Ion Binding to Carbonyl Oxygens by Solid-State 17O NMR, Gordon Research Conference, New London, CT, June 5-10 (2005)
  • Chekmenev, E.Y.; Hu, J.; Gor'kov, P.L.; Brey, W.W.; Cross, T.A.; Ruuge, A. and Smirnov, A.I., 15N and 31P solid-state NMR spectroscopy of M2 protein transmembrane domain in hydrated cylindrical lipid bilayers confined to nanopores, Biophysical Society 49th Annual Meeting, Long Beach, CA, February 12-16 (2005)
  • Chekmenev, E.Y.; Hu, J.; Gor’kov, P.L.; Brey, W.W.; Cross, T.A.; Poluektov, O.G.; Ruuge, A.; Alaouie, A.M. and Smirnov, A.I., Aligning Membrane Proteins and Peptides with Lipid Nanotube Arrays for Structural NMR and EPR Studies, 47th Rocky Mountain Conf. on Analytical Chemistry, Denver, CO, August (2005)
  • Cross, T.A.; Chapman, M.; Jap, B.; Chiu, W.; Loll, P.; Marshall, A.; Opella, S.; Sanders, C.; Sonnichsen, F.; Nakamoto, R.; Weiner, M.; Stauffacher, C. and Taylor, K., Membrane Protein Structural Genomics, 21st Int. Conf. on Magnetic Resonance in Biological Systems, Hyderabad, India, January (2005)
  • Fu, R.; Brey, W.W.; Shetty, K. and Cross, T.A., 900 MHz NMR at National High Magnetic Field Laboratory, Wuhan Institute of Physics and Mathematics, Wuhan, P. R. China, October 11-17 (2005)
  • Fu, R.; Chekmenev, E.Y. and Cross, T.A., Homonuclear Decoupling versus NMR Spin-locking in Polarization Inversion Spin Exchange at Magic Angle, 46th Experimental Nuclear Magnetic Resonance Conference (ENC), Providence, Rhode Island, April 10 - 15 (2005)
  • Fu, R.; Chekmenev, E.Y. and Cross, T.A., Solid State 15N/1H Chemical Shift Correlation Experiments of Aligned Samples at High Fields, 9th Beijing Conference and Exhibition on Instrumental Analysis (BCEIA), Beijing, P.R. China, October 19-23 (2005)
  • Moore, J.D.; Page, R.C.; Hua, Y.; Lee, S.; Opella, S. and Cross, T.A, Mycobacterium tuberculosis Integral Membrane Protein Rv1761c Backbone Assignment and Initial Structural Characterization, 49th Annual Biophysical Society Meeting, Long Beach, CA, February 12-16 (2005)
  • Moore, J.D.; Page, R.C.; Lee, S.; Opella, S.J. and Cross, T.A., Towards the structure determination of various alpha-helical membrane proteins from Mycobacterium tuberculosis, Emory University, Atlanta, GA, November 11-13 (2005)
  • Nguyen, H.B., and Cross, T.A., Expression, Purification and Initial Characterization of Several Integral Membrane Protein from Mycobacterium tuberculosis, Annual Biophysics Society Meeting, Long Beach, CA, February (2005)
  • Page, R.C. and Cross, T.A., Characterizing alpha-Helical Integral Membrane Proteins: Initial Results Towards a Joint Solution and Solid State NMR Based Structure Elucidation Scheme, 49th Annual Biophysical Society Meeting, Long Beach, CA, February 12-16 (2005)
  • Page, R.C.; Nguyen, H.; Moore, J.D.; Chase, R.; Gao, F.P.; Mobley, C.R.; Sanders, C.R.; Sonnichsen, F.D.; Lee, S.; Opella, S.J. and Cross, T.A., HSQC Spectra of Helical Integral Membrane Proteins: Sample Preparation Considerations and Advances in Spectral Quality, Emory University, Atlanta, GA, November 11-13 (2005)
  • Pearson, J.K.; Vijayvergiya, V.; Gao, P.F.; Cross, T.A. and Busath, D.D., Ammonium Conductance Properties of Influenza A M2 Channels in Lipid Bilayers: pH Dependence, Annual Biophysics Society Meeting, Long Beach, CA, February (2005)
  • Sharma, M. and Cross, T.A., Expression, Purification and Initial Characterization of the Integral Membrane Protein, Rv0150c from Mycobacterium tuberculosis by NMR Spectroscopy, Annual Biophysics Society Meeting, Long Beach, CA, February (2005)
  • Sharma, M. and Cross, T.A., Progress in Solution NMR based Structural Characterization o M2 Proton Channel from Influenza A Virus, ENC Conf., Asilomar, California, April 23 - 28 (2005)
  • Sharma, M. and Cross, T.A., Progress in Solution NMR based Structural Characterization of M2 Proton Channel from Influenza A Virus, ENC Conference, Providence, RI, April 10-15 (2005)
  • Strongin, B.A.; Vijayvergiya, V.; Gao, P.F.; Cross, T.A.; Davis, R.C. and Busath, D.D., Atomic Force Microscopy Imaging of Antibody-Bound Influenza A M2 Protein, Annual Biophysics Society Meeting, Long Beach, CA, February (2005)
  • Vijayvergiya, V.; Cardier, C.; Hu, J.; Cross, T.A. and Busath, D.D., Proton Conductance of the Influenza A M2 Transmembrane Peptide in Lipid Bilayers, Annual Biophysics Society Meeting, Long Beach, CA, February (2005)
  • Vijayvergiya, V.; Gao, P.F.; Cross, T.A. and Busath, D.D., Proton Conductance of 6F19 - Trp15,41 Influenza A Protein in Planar Lipid Bilayers, Annual Biophysics Society Meeting, Long Beach, CA, February (2005)

2004 Publications & Scientific Activities

    Peer-Reviewed Publications
  • Fu, R.; Hu, J. and Cross, T.A., Towards quantitative measurements in solid-state CPMAS NMR: A Lee-Goldburg frequency modulated cross polarization scheme, J. Magn. Reson., 168, 8-17 (2004) [read online]
  • Kim, H.; Cross, T.A. and Fu, R., Cross Polarization Schemes for Peptide Samples Oriented in Hydrated Phospholipid Bilayers, J. Magn. Reson., 168, 147-152 (2004) [read online]
  • Kim, S. and Cross, T.A., 2D Solid State NMR Spectral Simulations of 310, a, and r-Helices, J. Magn. Reson., 168, 187-193 (2004)
  • Mo, Y.; Cross, T.A. and Nerdal, W., Structural Restraints and Heterogeneous Orientation of the Gramicidin A Channel Closed State in Lipid Bilayers, Biophysical J., 86, 2837-2845 (2004)
  • Quine, J.R.; Cross, T.A.; Chapman, M.S. and Bertram, R., Mathematical aspects of protein structure determination with NMR orientational restraints, Bulletin of Mathematical Biology, 66, 1705-1730 (2004)
  • Schneider-Muntau, H.J.; Brandt, B.L.; Brunel, L.C.; Cross, T.A.; Edison, A.S.; Marshall, A.G. and Reyes, A.P., The National High Magnetic Field Laboratory, Physica B, 346-347, 643-648 (2004) [read online]
  • Vijayvergiya, V.; Chugg, N.; Swenson, M.; Gao, F.P.; Cross, T.A.and Busath, D.D., Conductance of Infuenza Virus M2 Proteins in Planar Lipid Bilayers, Biophysical J., 87 (3), 1697-1704 (2004)
  • Walian, P.; Cross, T.A. and Jap, B.K., Structural Genomics of Membrane Proteins, Genome Biology, 5, 215 (2004)
    Presentations, Posters & Abstracts
  • Chekmenev, E.Y.; Hu, J.; Waddell, K.W.; Gan, Z.; Wittebort, R.J. and Cross, T.A., Ion binding Study by 17O MAS NMR Spectroscopy at High Field and by Ab Initio Calculations in Model Peptide Systems, 15th Conf. Int. Society of Magnetic Resonance, Ponte Vedra Beach, FL, October 24-28 (2004) [read online]
  • Chekmenev, E.Y.; Page, R.C.; Korepanova, A. and Cross, T.A., Mycobacterium tuberculosis: solid state NMR study of Rv2433c protein, 45th Experimental Nuclear Magnetic Resonance Conf., Asilomar, CA, April 18-23 (2004) [read online]
  • Chekmenev, E.Y.; Shetty, K.K.; Falconer, N.D.; Gor'kov, P.L.; Brey, W.W. and Cross, T.A., Sample heat dissipation improvement in solid-state NMR of hydrated lipids, 5th Biennual Structural Biology Symposium, Tallahassee, FL, January 23-24 (2004)
  • Cross, T.A., “NMR Studies of the M2 Protein Channel from Influenza A Virus” – Membranes: A Challenge for Protein Magnetic Resonance, Tallahassee, FL, Janaury (2004)
  • Cross, T.A., Challenges in the Solid State NMR of Membrane Proteins, Rocky Mountain Conference, Denver, CO, August (2004)
  • Cross, T.A., Ion Interactions with the Gramicidin Channel and Proton Interactions with the M2 Channel, American Physical Society Annual Meeting, Montreal, Canada, March (2004)
  • Cross, T.A., Membrane Protein Expression, Sample Preparation, and Initial NMR Spectroscopy, Structural Proteomics of Integral Membrane Proteins, Marburg, Germany, September (2004)
  • Cross, T.A., The M2 Proton Channel from Influenza A Virus Structural and Functional Studies by Solid-State NMR, The 1st Pacific-Rim Int. Conf. on Protein Structure, Yokohama, Japan, April (2004)
  • Cross, T.A.; Busath, D.; Gao, F.P.; Hu, J. and Kim, S., Solid State NMR of a H+ Channel: from Structure to Function, Center for Membrane Proteomics Summerschool, Frankfurt, Germany, October (2004)
  • Cross, T.A.; Busath, D.; Zhou, H.-X.; Fu, R. and Gao, P.F., New Prospects, New Challenges with Membrane Protein Structure, Dynamics and Function, 15th Int. Society for Magnetic Resonance Meeting, Ponte Vedra Beach, FL, October (2004)
  • Cross, T.A.; Gao, F.P.; Korepanova, A.; Opella, S.J.; Sanders, C.; Nakomoto, R.; Marshall, A.; Taylor, K.; Jap, B.; Chiu, W.; Loll, P.; Chapman, M.; Stauffacher, C.; Wiener, M. and Sonnichsen, F., Membrane Protein Structural Genomics: A Multi-Technology Challenge, University of Virginia, Chalottesville, VA, March (2004)
  • Cross, T.A.; Nakamoto, R.K.; Jap, B.; Sanders, C.; Taylor, K.; Sonnichsen, F.; Opella, S.J and Gao, F.P., Membrane Protein Structural Genomics:Mycobacterium Tuberculosis, Structural Proteomics of Integral Membrane Proteins, Marburg, Germany, September (2004)
  • Cross, T.A.; Tian, C.; Wang, J.; Kim, S.; Pinto, L.H.; Tobler, K. and Lamb, R.A., Membrane Proteins: Challenges for Structural Biology by Solid State NMR, University of Delaware, DE, April (2004)
  • Fu, R.; Hu, J. and Cross, T.A., Towards quantitative measurements in solid-state CP/MAS NMR, 45th Experimental Nuclear Magnetic Resonance Conf. (ENC), Pacific Grove, CA, April 17-23 (2004)
  • Fu, R.; Hu, J.; Li, C. and Cross, T.A., Solid State NMR Study of Membrane Proteins Oriented in Hydrated Lipid Bilayers, Int. Biomolecular NMR Symposium, Wuhan, P.R. China, June 28- July 1 (2004)
  • Fu, R.; Li, C.; Hu, J.; Gao, F. and Cross, T.A., Polarization Inversion Spin Exchange at the Magic Angle and its Applications to Membrane Proteins oriented in Hydrated Phospholipid Bilayers, Shanghai Institute of Materia Medica, the Chinese Academy of Sciences, Shanghai, P.R. China, June 21-22 (2004)
  • Gao, F.P.; Qin, H.; Hua, Y.; Sharma, M. and Cross, T.A., High-Throughput Cloning and Over-Expression of Integral Membrane Protein from Mycobacterium Tuberculosis for NMR Structural Genomics Studies, Int. Workshop on Structural Proteomics of Integral Membrane Proteins, Schloss Rauischholzhaussen, Marburg, Germany, September 27-30 (2004)
  • Korepanova, A. and Cross, T.A., Overexpression and Purification of Membrane Proteins from Mycobacterium tuberculosis in Escherichia coli as Fusions to maltose Binding Protein, 5th Biannual Structural Biology Symposium; Membranes: a Challenge for Protein Magnetic Resonance, Florida State University, Tallahassee, FL, January 23 -24 (2004)
  • Li, C.; Mo, Y.; Gao, P.; Hu, J.; Chekmenev, E.Y.; Gor'kov, P.L.; Brey, W.W. and Cross, T.A., Analysis of RF Heating and Sample Stability in Aligned Static Solid-State NMR Spectroscopy, 45th ENC, Pacific Grove, CA, April 18-23 (2004)
  • Moore, J.; Korepanova, A. and Cross, T.A., Expression, Purification, and Initial Characterization of Rv2719c, Rv1924c, and Rv1239c Integral Membrane Proteins of Mycobacterium tuberculosis, 5th Biennial Structural Biology Symposium; Membranes: a Challenge for Protein Magnetic Resonance, Florida State University, Tallahassee, FL, January 23-24 (2004)
  • Moore, J.; Korepanova, A. and Cross, T.A., Expression, Purification, and Initial Structural Characterization of Mycobacterium tuberculosis Membrane Proteins, 48th Annual Biophysical Society Meeting, Baltimore, MD, February 14-18 (2004); Published in Biophysical Journal, 86 (1), 98 (2004)
  • Moore, J.D.; Korepanova, A. and Cross, T.A., Purification and Characterization of Mycobacterium tuberculosis Integral Membrane Proteins, January (2004); Published in National High Magnetic Field Laboratory 2003 Annual Research Review, 33-34 (2004)
  • Moore, J.D.; Korepanova, A.; Hau, Y.; Qin, H.; Gao, F.; Lee, S.; Opella, S. and Cross, T.A., Initial Characterization of Mycobacterium tuberculosis integral membrane proteins Rv2719c, R1761c, and Rv0008 expressed in E. coli, Int. Society for Magnetic Resonance, Ponte Vedra Beach, FL, October 24-28 (2004)
  • Nguyen, H.B.; Moore, J.D.; Korepanova, A. and Cross, T.A., Expression, Purification, and Initial Characterization of Two Membrane Proteins: Rv0476s and Rv1824 from Mycobacterium tuberculosis, Int. Society for Magnetic Resonance, Ponte Vedra Beach, FL, October 24-28 (2004)
  • Nozirov, F.; Gao, P.; Witter, R.; Fu, R.; Ulrich, A. and Cross, T. A., High field 19F solid-state NMR spectroscopy for structural and conformational study of M2 membrane protein, 15th Triennial Conf. for the Int. Society for Magnetic Resonance (ISMAR), Ponte Vedra Beach, Jacksonville, FL, October 24-28 (2004); Published in ISMAR Book of Abstracts, 245 (2004)
  • Page, R.C.; Chekmenev, E.Y.; Lee, S.; Opella, S.J. and Cross, T.A., Structural Characterization of the Integral Membrane Protein Rv2433c from Mycobacterium tuberculosis by Solution and Solid State NMR Spectroscopy, 15th Conf. Int. Society of Magnetic Resonance, Ponte Vedra Beach, FL, October 24-28 (2004)
  • Page, R.C.; Korepanova, A. and Cross, T.A., Expression, Purification, and Initial Characterization by NMR Spectroscopy of the Integral Membrane Protein Rv2433c from Mycobacterium Tuberculosis, 5th Biennial Structural Biology Symposium; Membranes: A Challenge for Protein Magnetic Resonance, Turnbull Conference Center, Florida State University, Tallahassee, FL, January 23-24 (2004)
  • Page, R.C.; Korepanova, A. and Cross, T.A., Expression, Purification, and Initial Characterization by NMR Spectroscopy of the Integral Membrane Protein Rv2433c from Mycobacterium tuberculosis, 48th Annual Biophyiscal Society Meeting, Baltimore Convention Center, Baltimore, MD, February 14-18 (2004); Published in Biophysical J., 86 (1), 98 (2004)
  • Saha, S.; Chekmenev, E.Y.; Li, C.; Gor'kov, P.L.; Cross, T.A. and Brey, W.W., Faraday Shielding of Solid State NMR Samples, ISMAR, October 24-28 (2004)
  • Xiong, Y.; Chalmers, M.J.; Gao, F.P.; Cross, T.A. and Marshall, A.G., ESI FT-ICR and LC-LTQ Mass Spectrometry Identification of Mycobacterium tuberculosis H37Rv Membrane Proteins, 52nd American Society for Mass Spectrometry Annual Conference on Mass Spectrometry and Allied Topics, Nashville, TN, May 23-27 (2004)

2003 Publications & Scientific Activities

    Peer-Reviewed Publications
  • Bertram, R.; Asbury, T.; Fabiola, F.; Quine, J.R.; Cross, T.A. and Chapman, M.S., Atomic Refinement with Correlated Solid-State NMR Restraints, J. Magn. Reson., 163 (2), 300-309 (2003) [read online]
  • Bingham, N.C.; Smith, N.E.C.; Cross, T.A. and Busath, D.D., Molecular Dynamics Simulations of Trp Side-chain Conformational Flexibility in the Gramicidin A Channel, Biopolymers, 71, 593+600 (2003) [read online]
  • Tian, C.; Gao, P.F.; Pinto, L.H.; Lamb, R.A. and Cross, T.A., Initial Structure and Dynamic Characterization of the M2 Protein Transmembrane and Amphipathic Helices in Lipid Bilayers, Protein Science, 12, 2597-2605 (2003) [read online]
    Presentations, Posters & Abstracts
  • Bertram, R.; Asbury, T.; Fabiola, F.; Quine, J.R.; Chapman, M.S. and Cross, T.A., Atomic Refinement Using Correlated Orientational Data from Solid-State NMR, Annual Biophysics Society Meeting, San Antonio, TX, March (2003)
  • Fu, R.; Hu, J. and Cross, T.A., Lee-Goldburg Frequency Modulated Cross Polarization in Solid-State CPMAS NMR, 33rd Southeastern Magnetic Resonance Conf., Tallahassee, FL, October 17-19 (2003)
  • Fu, R.; Li, C.; Kim, H.; Hu, J. and Cross, T.A., Solid State NMR Experiments of Aligned Samples, The 3rd Alpine Conf. on Solid State NMR, Chamonix-Mont Blanc, France, September 14-18 (2003)
  • Gan, Z.; Gor'kov, P.; Cross, T.; Kwak, H-T.; Brey, W.; Massiot, D.; Samoson, A.; Butler, L.; Prasad, S. and Grandinetti, P., Practical Aspect and Recent Results of High Resolution Solid State NMR of Quadrupolar Nuclei Using 45 T Hybrid Magnet, 44th Experimental NMR Conf., Savannah, GA, March (2003) [read online]
  • Gao, F.; Hua, Y.; Korepanopva, A.; Peskova, Y.; Rohal, K.; Nakamoto, R.K. and Cross, T.A., Membrane Protein Structural Genomics: Mycobacterium Tuberculosis, Annual Biophysics Society Meeting, San Antonio, TX, March (2003)
  • Gao, F.; Mo, Y.; Tian, C.; Li, C. and Cross, T.A., Solid State NMR Studies of Membrane Associated Ion Channels, Annual Biophysics Society Meeting, San Antonio, TX, March (2003)
  • Hu, J.; Nishimura, K.; Fu, R. and Cross, T.A., Exploring the Binding Effect of Amantadine on the Histidine Side Chain and Backbone of the M2 Protein Transmembrane Segment from Influenza A Virus by Solid State NMR, Biophysics Society Conf., San Antonio, TX, 6 (2003); Published in Biophysical J., 82 (1), 1 (2002) [read online]
  • Hu, J.; Nishimura, K.; Zhang, L.; Fu, R. and Cross, T.A., Exploring the Binding Effect of Amantadine on the Histidine Side Chain and Backbone of the M2 Protein Transmembrane Segment from Influenza A Virus by Solid State NMR, 44th Experimental Nuclear Magnetic Resonance Conf., Savannah, GA, March 30-April 4 (2003)
  • Hu, J.; Nishimura, K.; Zhang, L.; Fu, R. and Cross, T.A., Histidine Protonation in the Transmembrane Segment of the M2 Proton Channel from Influenza A Virus, 33rd Southeastern Magnetic Resonance Conf., NHMFL, Tallahassee, FL, October 17-19 (2003)
  • Hu, J.; Wang, J.; Kim, S.; Tian, C.; Quine, J.R. and Cross, T.A., All About Orientation: The Road to Membrane Protein Structure Determination by Solid-State NMR, Gordon Conf., Newport, RI, 5 (2003)
  • Kim, H.; Fu, R. and Cross, T.A., Cross Polarization Schemes for Peptide Samples Oriented in Hydrated Phospholipid Bilayers, 44th Experimental Nuclear Magnetic Resonance Conf. (ENC), Savannah, GA, March 30 - April 4 (2003)
  • Korepanova, A.; Gao, P.; Hua, Y.; Peskova, Y.; Rohal, K.; Nakamoto, R.K. and Cross, T.A., Membrane Proteins Structural Genomics: Mycobacterium Tuberculosis, 47th Annual Biophysical Society Meeting, San Antonio, TX, March 1-5 (2003); Published in Biophysical Journal, 84 (2), 349 (2003)
  • Li, C.; Hu, J.; Tian, C.; Fu, R.; Gao, F.; Mo, Y.; Gor'kov, P.; Brey, W. and Cross, T.A., Sensitivity Improvements in Membrane Protein Solid State NMR Experiments, Annual Biophysics Society Meeting, San Antonio, TX, March (2003)
  • Li, C.; Mo, Y.; Hu, J.; Fu, R.; Gao, F.; Tian, C.; Gor'kov, P.; Brey, W.W. and Cross, T.A., Improvements in Membrane Protein PISEMA Solid State NMR Experiments, 44th Experimental NMR Conf., Savannah, GA, March 30 – April 4 (2003)
  • Nguyen, H.B.; Gao, P. and Cross, T.A., Semisynthesis of the Influenza Virus M2 Ion Channel Protein Using a Mycobacterium xenopi GyrA Intein Expression System without Substituting Amino Acid at Cleavage Site, 33rd Southeastern Magnetic Resonance Conference, Tallahassee, FL, October 17-19 (2003)
  • Page, R.C.; Moore, J.; Korepanova, A. and Cross, T.A., Expression, Purification, and Initial Characterization by NMR Spectroscopy of Multiple Integral Membrane Proteins from Mycobacterium tuberculosis, 33rd Southeastern Magnetic Resonance Conf., Tallahassee, FL, October 17-19 (2003)
  • Quine, J.R.; Srinivasan, P.; Achuthan, S.; Bertram, R.; Chapman, M. and Cross, T.A., Analysis of Orientational Restraints in NMR, Annual Biophysics Meeting, San Antonio, TX, March (2003)
  • Schneider-Muntau, H.J.; Brandt, B.L.; Brunel, L.-C.; Cross, T.A. and Marshall, A.G., The National High Magnetic Field Laboratory, 7th Int. Symposium on Research in High Magnetic Fields, Toulouse, France, July (2003)
  • Vijayvergiya, V.; Cross, T.A. and Busath, D.L., Ion Channel Studies of Influenza Virus M2 in Planar Lipid Bilayers, Annual Biophysics Society Meeting, San Antonio, TX, March (2003)
    Books, Chapters, Reviews & Other One-Time Publications
  • Quine, J.R. and Cross, T.A., "Protein Structure from Solid-State NMR", in Mathematical Methods for Protein Structure Analysis and Design (Guerra and Istral eds.), Lecture Notes in Bioinformatics, Springer-Verlag, 2666, 131-136, 2003.

2002 Publications & Scientific Activities

    Peer-Reviewed Publications
  • Cole, C.D.; Frost, A.S.; Thompson, N.; Cotten, M.; Cross, T.A. and Busath, D.D., Non-contact dipole effects on channel permeation. VI. 5F- and 6F-Trp gramicidin channel currents, Biophysical J., 83, 1974-1986 (2002)
  • Fu, R.; Tian, C. and Cross, T.A., NMR spin locking of proton magnetization under frequency switched Lee-Goldburg (FSLG) pulse sequence, J. Magn. Reson., 154, 130-135 (2002) [read online]
  • Fu, R.; Tian, C.; Kim, H.; Smith, S.A. and Cross, T.A., The effect of Hartmann-Hahn mismatching on polarization inversion spin exchange at the magic angle, J. Magn. Reson., 159, 167-174 (2002) [read online]
  • Gan, Z.; Gor'kov, P.; Cross, T.A.; Samosan, A. and Massiot, D., Seeking for Higher Resolution and Sensitivity for NMR of Quadrupolar Nuclei at Ultra High Magnetic Fields, J. Am. Chem. Soc., 124 (20), 5634-5935 (2002) [read online]
  • Gowan, J.A.; Markham, J.C.; Morrison, S.E.; Cross, T.A.; Busath, D.D.; Maples, E.J. and Schumaker, M.F., The Role of Trp Side Chains in Tuning Single Proton Conduction through Gramicidin Channels, Biophysical J., 83, 880-898 (2002) [read online]
  • Kim, S. and Cross, T.A., Uniformity, Ideality and Hydrogen Bonds in Transmembrane a-Helices, Biophysical J., 83, 2084-2095 (2002) [read online]
  • King, B.; Cross, T. and Gan, Z., Using solid state 0-17 NMR to study humic acids, ACS Abstracts of Papers, 223, 656 (2002)
  • Nishimura, K.; Kim, S.; Zhang, L. and Cross, T.A., The Closed State of a H+ Channel Helical Bundle: Combining Precise Orientational and Distance Restraints from Solid State NMR, Biochemistry, 44, 13170-13177 (2002)
  • Tian, C.; Tobler, K.; Pinto, L.H.; Lamb, R. and Cross, T.A., Expression and Initial Structural Insights from Solid State NMR of the M2 Proton Channel from Influenza A Virus, Biochemistry, 41, 11294-11300 (2002)
    Presentations, Posters & Abstracts
  • Brey, W.W.; Gor'kov, P.L.; Fu, R.; Tian, C. and Cross, T.A., Improvements in NMR Probes for Membrane Protein Studies, 43rd Experimental NMR Conf., Pacific Grove, CA, April 14-19 (2002)
  • Cross, T.A.; Edison, A.S. and Logan, T.M., NMR Spectroscopy and Imaging Facility, National High Magnetic Field Laboratory, Annual Experimental NMR Conf., Asilomar, CA, April (2002)
  • Cross, T.A.; Gao, F.; Korepanova, A.; Hua, Y.; Peskova, Y.B. and Nakamoto, R., Integral Membrane Protein Structural Genomics: Mycobacterium tuberculosis, ISGO Int. Conf. on Structural Genomics, Berlin, Germany, October (2002)
  • Cross, T.A.; Tian, C.; Gao, F.; Nishimura, K.; Kim, S.; Hu, J.; Fu, R.; Tobler, K.; Pinto, L.H. and Lamb, R.A, Advances in PISEMA Spectroscopy of Membrane Proteins, 20th Int. Conf. on Magnetic Resonance in Biological Systems, Toronto, Canada, August (2002)
  • Cross, T.A.; Tian, C.; Gao, F.; Nishimura, K.; Kim, S.; Tobler, K.; Pinto, L.H. and Lamb, R.A., Initial Structure of the M2 Proton Channel from Influenza A Virus by Solid State NMR, 46th Annual Meeting of the Biophysical Society, San Francisco, CA, February (2002)
  • Cross, T.A.; Tian, C.; Wang, J.; Kim, S.; Pinto, L.H.; Tobler, K. and Lamb, R.A., Membrane Protein Structural Elucidation: PISA Wheels from Solid State NMR Spectroscopy, University of Minnesota, Minneapolis, MN, April (2002)
  • Cross, T.A.; Tian, C.; Wang, J.; Kim, S.; Pinto, L.H.; Tobler, K. and Lamb, R.A., Membrane Protein Structure from Solid-State NMR: PISA Wheels, Ohio University, Athens, OH, May (2002)
  • Fu, R.; Kim, H.; Li, C.; Tian, C. and Cross, T.A., Cross Polarization Schemes in Polarization Inversion Spin Exchange at the Magic Angle (PISEMA), 31st Southeastern Magnetic Resonance Conf. (SEMRC), Raleigh, NC, October 24-27 (2002)
  • Hu, J. and Cross, T.A., Histidine Residue pH Titration and Amantadine Inhibition Studies of M2 Protein from Influenza A Virus by Magic Angle Spinning (MAS) NMR, Annual Biophysical Society Meeting, San Francisco, CA, February (2002)
  • Hu, J.; Nishimura, K. and Cross, T.A., Solid State NMR Spectroscopy of Histidine-37 in the Proton Channel Formed by the Transmembrane Peptide, M2-TMP, Annual Experimental NMR Conf., Asilomar, CA, April (2002)
  • Kim, S. and Cross, T.A., Conformational Analysis and NMR Spectral Simulation of 310, a, and p Helices, Annual Biophysical Society Meeting, San Francisco, CA, February (2002)
  • Kim, S.; Nishimura, K.; Wang, J.; Porges, A.; Ku, S.; Quine, J.R. and Cross, T.A., Refined Molecular Model Structure Based on Solid State NMR Orientational Restraints: A Tetramer Model Structure of Influenza M2 H+ Channel Transmembrane Region, Annual Biophysical Society Meeting, San Francisco, CA, February (2002)
  • Kim, S.; Tian, C.; Kim, H. and Cross, T.A., Direct Observation of Helical Geometry and Resonance Assignment in 2D Solid State NMR Spectra, Annual Experimental NMR Conf., Asilomar, CA, April (2002)
  • Korepanova, A. and Cross, T.A., Mycobacterium tuberculosis Membrane Protein Production (invited talk), Third TB Structural Genomics Consortium Retreat, Santa Fe, NM, September 8 -10 (2002)
  • Tian, C.; Gao, F.; Li, C. and Cross, T.A., Transmembrane Helix Backbone Structural Characterization of M2 Proton Channel from Influenza A Virus Using 2D PISEMA Solid State NMR, Annual Experimental NMR Conf., Asilomar, CA, April (2002)
  • Tian, C.; Gao, F.; Tobler, K.; Pinto, L.H.; Lamb, R.A. and Cross, T.A., Structural Characterization of M2 Protein from Influenza A Virus Using Solution NMR and Solid State NMR, Annual Biophysical Society Meeting, San Francisco, CA, February (2002)

2001 Publications & Scientific Activities

    Peer-Reviewed Publications
  • Anderson, D.G.; Shirts, R.A.; Cross, T.A. and Busath, D.D., Non-contact dipole effects on channel permeation. V. Computed potentials for fluorinated gramicidin, Biophysical J., 81, 1255-1264 (2001)
  • Denny, J.K.; Wang, J.; Cross, T.A. and Quine, J.R., PISEMA Powder Patterns and PISA Wheels, J. Magn. Reson., 152, 217-226 (2001)
  • Kim, S.; Quine, J.R. and Cross, T.A., Complete Cross-Validation and R-Factor Calculation of a Solid-State NMR Derived Structure, J. Am. Chem. Soc., 123, 7292-7298 (2001)
  • Markham, J.C.; Gowen, J.A..; Cross, T.A. and Busath, D.D., Comparison of Gramicidin A and Gramicidin M Channel Conductance Dispersities, Biochim. Biophys. Acta, 1513, 185-192 (2001)
  • Nishimura, K.; Fu, R. and Cross, T.A., The Effect of rf Inhomogeneity on Heteronuclear Dipolar Recoupling in Solid State NMR; Practical Performance of SFAM and REDOR, J. Magn. Reson., 152, 227-233 (2001)
  • Thompson, N.; Thompson, G.; Cole, C.C.; Cotten, M.; Cross, T.A. and Busath, D.D., Non-contact dipole effects on channel permeation. IV. Kinetic model of 5F-Trp13 gramicidin A currents, Biophysical J., 81, 1245-1254 (2001)
  • Wang, J.; Kim, S.; Kovacs, F. and Cross, T.A., M2 Protein H+ Channel: A Refined Backbone Structure for the Transmembrane Region, Protein Science, 10, 2241-2250 (2001)
    Presentations, Posters & Abstracts
  • Bertram, R.; Kim, S.; Quine, J.R.; Xu, M.; Chapman, M.S. and Cross, T.A., Molecular Refinement and Cross-Validation with Solid State NMR Orientational Data, Annual Biophysical Society Meeting, Boston, MA, February (2001)
  • Bertram, R.; Quine, J.R.; Kim, S.; Chapman, M.S. and Cross, T.A., Molecular Refinement and Cross-Validation with Solid State NMR Orientational Data, Annual Experimental NMR Conference, Orlando, FL, March (2001)
  • Cross, T.A., High Performance High Field NMR in Resistive Magnets, 2nd Annual Meeting of the High Field Infrastructure Cooperative Network, Nijmegen, The Netherlands, September (2001)
  • Cross, T.A.; Kim, S.; Bertram, R.; Chapman, M. and Quine, J., Molecular Interactions and Structural Refinement in a Membrane Environment, Computational Aspects – Biomolecular NMR, Il Ciocco, Italy, May (2001)
  • Cross, T.A.; Kim, S.; Wang, J.; Bertram, R. and Quine, J.R., From Topology to Cross-Validation of Macromolecular Structures by Solid-State NMR, Annual Meeting of the Polymer Society of Japan, Tokyo, May (2001)
  • Cross, T.A.; Kim, S.; Wang, J.; Bertram, R.; Chapman, M. and Quine, J., From Topology to Refinement and Cross-Validation of Membrane Bound Macromolecules by Solid-State NMR, Rocky Mountain Conference on Analytical Chemistry, Denver, CO, August (2001)
  • Cross, T.A.; Tian, C.; Gao, F.; Nishimura, K.; Kim, S.; Tobler, K.; Pinto, L.H. and Lamb, R.A., Initial Structure of the M2 Protein Channel from Influenza A Virus by Solid State NMR, The 46th Annual Meeting of the Biophysical Society, San Francisco, February (2001)
  • Cross, T.A.; Tian, C.; Wang, J.; Kim, S.; Pinto, L.H.; Tobler, K. and Lamb, R.A., Towards a High Resolution Structure of the M2 Proton Channel in Influenza A Virus Using Solid-State NMR, Int. Society of Magnetic Resonance, Rhodes, Greece, August (2001)
  • Cross, T.A.; Tian, C.; Wang, J.; Kim, S.; Pinto, L.H.; Tobler, K. and Lamb, R.A., Towards a Three Dimensional Structure of the M2 Proton Channel from Influenza A Virus Using Solid-State NMR of Aligned Liquid Crystalline Lipid Bilayers, Rockefeller University, Rockefeller, New York, November (2001)
  • Cross, T.A.; Tian, C.; Wang, J.; Kim, S.; Quine, J.; Gao, F.; Pinto, L.H.; Tobler, K. and Lamb, R.A., PISA Wheels: Solid State NMR Topology Elucidation of Membrane Protein Structure, The 1st International Meeting on Proton Channels, Villars, Switzerland, September (2001)
  • Cross, T.A.; Tian, C.; Wang, J.; Kim, S.; Quine, J.; Gao, F.; Pinto, L.H.; Tobler, K. and Lamb, R.A., PISA Wheels: Solid State NMR Topology Elucidation of Membrane Proteins, The 2nd Alpine Conference on Solid State NMR, Chamonix-Mont Blanc, France, September 9-13 (2001)
  • Denny, J.K.; Wang, J.; Cross, T.A. and Quine, J.R., PISEMA Powder Patterns and PISA Wheels, Annual Biophysical Society Meeting, Boston, MA, February (2001)
  • Fu, R.; Tian, C. and Cross, T.A., NMR Spin Locking Magnetization under Frequency Switched Lee-Goldberg (FSLG) Pulse Sequence, Annual Experimental NMR Conference, Orlando, FL, March (2001)
  • Fu, R.; Tian, C. and Cross, T.A., NMR Spin Locking under Frequency-Switched Lee-Goldburg (FSLG) Pulse Sequences, 9th Beijing Conference and Exhibition on Instrumental Analysis (BCEIA), Beijing, P.R. China, October 17-20 (2001)
  • Gan, Z.; Gor'kov, P.; Cross, T.; Samoson, A.; Huguenard, C.; Taulelle, F.; Massiot, D.; Mrse, A.; Butler, L. and Hoatson, G., High field fast-speed and satellite transition MAS NMR of quadrupolar nuclei, 43rd Rocky Mountain Conference on Analytical Chemistry, Denver, Colorado, July 29-August 2 (2001)
  • Gan, Z.; Gor'kov, P.; Cross, T.; Samoson, A.; Massiot, D. and Butler, L, High Field Fast-MAS NMR of Quadrupolar Nuclei up to 40 kHz and 40 T, SouthEastern Magnetic Resonance Conference, Gainesville, FL, October 26-28 (2001)
  • Gao, F.; Tian, C. and Cross, T.A., Solid State NMR Observation of Phenylalanine Residues in M2 Protein from Influenza A Virus, The International Symposium on the Progress in Magnetic Resonance Methodologies, Wuhan, China, October 22-23 (2001)
  • Hu, J.; Tian, C.; Gao, F and Cross, T.A., Solid-State NMR Structural Studies of M2 Protein, A Selective Proton Channel in Influenza A Virus, Southeastern Magnetic Resonance Conference, Gainesville, FL, October 26-28 (2001)
  • Hu, J.; Wang, J. and Cross, T.A., Imaging Membrane Protein Helical Wheels, Southeastern Magnetic Resonance Conference, Gainesville, FL, October 26-28 (2001)
  • Hu, J.; Wang, J.; Kim, S.; Tian, C. and Cross, T.A., Imaging Membrane Protein Helical Wheels, Membrane Protein Structure/Function Keystone Conference, Tahoe City, March (2001)
  • Hu, J.; Wang, W.; Denny, J.; Kim, S. and Cross, T.A., Structural Determination of the M2 Transmembrane Peptide (TMP) H+ Channel from Solid State NMR, Keystone Conference on Membrane Protein Structure/Function, Tahoe City, March (2001)
  • Kim, S. and Cross, T.A., Solid State NMR Structure Determination of Membrane Protein Helices, Southeastern Magnetic Resonance Conference, Gainesville, FL, October (2001)
  • Kim, S.; Nishimura, K. and Cross, T.A., Solid State NMR Structure Determination of Hydrogen Bonding and Uniformity of Membrane Protein Helices, Annual Experimental NMR Conference, Orlando, FL, March (2001)
  • King, B. and Cross, T.A., Using Nuclear Magnetic Resonance Spectroscopy to Study the Structure of Humic Acid, Annual Experimental NMR Conference, Orlando, FL, March (2001)
  • Markham, J.C.; Merrell, J.; Cross, T.A. and Busath, D.D., A Discrete Conductance Substate in Gramicidin M. Channels, Annual Biophysical Society Meeting, Boston, MA, February (2001)
  • Mo, Y.; Tian, C.; Hu, J.; Wang, J. and Cross, T.A, The Structural Studies of Gramicidin A in long Lipid Bilayers, Annual Biophysical Society Meeting, Boston, February (2001)
  • Mo, Y.; Tian, C.; Hu, J.; Wang, J.; Nishimura, K. and Cross, T.A., Structural Studies of the Gramicidin A Monomer in Long Chain Lipid Bilayers, Annual Experimental NMR Conference, Orlando, March (2001)
  • Nishimura, K.; Kim, S. and Cross, T.A., The structural determination of transmembrane region of M2 protein from influenza virus A in lipid bilayer based on accurate distance measurement by solid state NMR, Biophysical Society of Japan Annual Meeting, Osaka, Oct. (2001)
  • Nishimura, K.; Fu, R. and Cross, T.A., The Effect of rf Inhomogeneity on Heteronuclear Dipolar Recoupling in Solid State NMR; Practical Performance of SFAM and REDOR, 40th Japan NMR Conference, Kyoto, Japan, Nov. 14-16 (2001)
  • Nishimura, K.; Kim, S.; Tian, C.; Wang, J.; Tobbler, K.; Lamb, R. and Cross, T.A., Experimental Evidence for the Bundle Structure of M2 H+ Channel Transmembrane Peptide(TMP) and Intact Protein from Influenza A Virus from Solid State NMR, 45th Biophysical Society Annual Meeting, Boston, MA, Feb. 17-21 (2001)
  • Nishimura, K.; Kim, S.; Wang, J. and Cross, T.A., Experimental Evidence for the Bundle Structure of M2H+ Channel Transmembrane Peptide(TMP) from Solid State NMR Distance Measurements., 42nd Experimental NMR Conference, Orlando, March 11-16 (2001)
  • Painter, T.A.; Dixon, I.; Swenson, C.; Marshall, S.; Markiewicz, D.; Brey, W.W.; Cross, T. and Ferner, J., Wide Bore 900 MHz NMR System Fabrication and Testing, 42nd Experimental NMR Conference, Orlando, FL, March 11-16 (2001)
  • Quine, J.R. and Cross, T.A, Transmembrane protein structure from NMR, mathematical analysis, National American Mathematical Society Meeting, New Orleans, LA, January (2001)
  • Schumaker, M.F.; Gowen, J.A.; Arkham, J.C.; Morrison, S.E.; Cross, T.A. and Busath, D.D., A Unified Model of Proton Conductance through Gramicidin A, B and M, Annual Biophysical Society Meeting, Boston, MA, February (2001)
  • Thompson, K.; Duffin, R.; Garrett, M.; Woodbury, J.W. and Cross, T.A., Gramicidin A Conductance is Enhanced by Phloretin in Phospholipid but not Monoglyceride Bilayers, Annual Biophysical Society Meeting, Boston, MA, February (2001)
  • Tian, C. and Cross, T.A., Initial Structural Characterization of M2 Proton Channel from Influenza A virus using Solid State NMR, International Symposium of Magnetic Resonance Methodology, WuHan, P.R. China, November (2001)
  • Tian, C. and Cross, T.A., Membrane Protein Structural Characterization using Solid State Nuclear Magnetic Resonance, Shanghai Jiao University, Shanghai, P.R. China, October (2001)
  • Tian, C.; Tobbler, K.; Lamb, R. and Cross, T.A., Structural Insight of Intact M2 Membrane Protein from Influenza A Virus by 2D PISEMA, Annual Experimental NMR Conference, Orlando, FL, March (2001)
    Books, Chapters, Reviews & Other One-Time Publications
  • Cross, T.A.; Kim, S.; Wang, J. and Quine, J.R., "From Topology to High Resolution Membrane Protein Structures", Proceedings of the Workshop on The Future of Solid State NMR in Biology, 55-70, 2001.

2000 Publications & Scientific Activities

    Peer-Reviewed Publications
  • Bertram, R.; Quine, J.R.; Chapman, M. and Cross,T.A., Atomic Refinement Using Orientational Restraints from Solid-State NMR, J. Magn. Reson., 147, 9-16 (2000)
  • Byström, T.; Strandberg, E.; Kovacs, F.A.; Cross, T.A. and Lindblom, G., Influence of Transmembrane Peptides on Bilayers of Phosphatidylcholines with Different Acyl Chain Lengths Studied By Solid-State NMR, Biochim. Biophys. Acta, 1509, 335-345 (2000)
  • Cross,T.A. and Quine, J.R., Protein Structure In Anisotropic Environments: Development of Orientational Constraints, Concepts in NMR, 12, 55-70 (2000)
  • Fu, R.; Cotten, M. and Cross, T.A., Inter- and Intramolecular Distance Measurement by Solid-State MAS NMR: Determination of Gramicidin A Channel Dimer Structure in Hydrated Phospholipid Bilayers, J. Biomol. NMR, 16, 261-268 (2000)
  • Kovacs, F.A.; Denny, J.K.; Song, Z.; Quine, J.R. and Cross,T.A., Heliz Tilt of the M2 Transmembrane Protein: An Intrinsic Property of the Protein, J. Mol. Biol., 295, 117-125 (2000)
  • Quine,J.R. & Cross,T.A., Protein Structure in Anisotropic Environments: Unique Structural Fold from Orientational Constraints, Concepts in NMR, 12, 71:82 (2000)
  • Soghomonian, V.; Sabo, M.; Powell, A.; Murphy, P.; Rosanske, R.; Cross, T.A. and Schneider-Muntau, H.J., Identification and minimization of sources of temporal instabilities in high field (>23 T) resistive magnets, Rev. Sci. Instrum., 71 (7), 2882-2889 (2000)
  • Song, Z.; Kovacs, F.A.; Wang, J.; Denny, J.; Schekar, S.C.; Quine, J.R. & Cross, T.A., Transmembrane Domain of M2 Protein from Influenza A Virion as Studied by PISEMA in Solid-State 15 N NMR, Biophysical J., 79, 767-775 (2000)
  • Wang, J.; Denny, J.K.; Tian, C.; Kovacs, F.; Song, Z.; Fu, R.; Quine, J.R.; Cross, T.A.; Kim, S.; Mo, Y.; Nishimura, K. & Gan, Z., Imaging Membrane Protein Helical Wheels, J. Magn. Reson., 144, 162-167 (2000) [read online]
    Presentations, Posters & Abstracts
  • Cole, C.; Thomson, N.; Frost, A.; Nelson, P.,Cotten, M.; Cross, T.A.; Busath, D., Current-Voltage Relationships of 5- and 6-F Trp Analogs of Gramicidin A, Annual Biophysical Society Meeting, New Orleans, LA, February (2000)
  • Cross, T.A, Chemistry and Biology at the Frontiers of High Field NMR Spectroscopy, Department of Chemistry, Florida Institute of Technology, January (2000)
  • Cross, T.A., Blackband, S., Logan, T., Murali, N., Fu,R., Gan, Z., Smith, S., NMR Spectroscopy and Imaging User Facility, National High Magnetic Field Laboratory, Experimental NMR Conference, Asilomar, CA, March (2000)
  • Cross, T.A., Tian, C., Wang, J., Kim, S. & Quine, J., Imaging Membrane Protein Helical Wheels by Solid State NMR Spectroscopy: M2 Protein from Influenza A, Programs of Structural Biology and Molecular Biophysics, Univ. of Pittsburg, Pittsburg, PA, November (2000)
  • Cross, T.A., Wang, J., Tin, C., Kim, S., Kovacs, F., Mo, Y., Nishimura, K., Gan, Z., Fu, R., Song, Z., Denny, J. & Quine, J, Imaging membrane Protein Helical Wheels, Tokyo University of Agriculture & Technology, Tokyo, Japan, March (2000)
  • Cross, T.A., Wang, J., Tin, C., Kim, S., Kovacs, F., Mo, Y., Nishimura, K., Gan, Z., Fu, R., Song, Z., Denny, J. & Quine, J., Imaging membrane Protein Helical Wheels, Himeji Institute of Technology, Harima Science Garden City, Japan, March (2000)
  • Cross, T.A., Wang, J., Tin, C., Kim, S., Kovacs, F., Mo, Y., Nishimura, K., Gan, Z., Fu, R., Song, Z., Denny, J. & Quine, J., Imaging membrane Protein Helical Wheels, University of Kobe, Kobe, Japan, March (2000)
  • Cross, T.A.; Kim, S.; Wang, J.; Quine, J.R., From Topology to High Resolution Membrane Protein Structures, University of Leiden Workshop on the Future of Solid State NMR in Biology, Leiden, The Netherlands, October (2000)
  • Cross, T.A.; Wang, J.; Tin, C.; Kim, S., Kovacs, F.; Mo, Y.; Nishimura, K.; Gan, Z.; Fu, R.; Song, Z,; McNiel, S.; Xu, M.; Bond, H.; Denny, J. and Quine, J., Three-Dimensional Protein Structures: Mathematical and Technical Challenges, Florida State University, Department of Mathematics, Tallahassee, FL, March (2000)
  • Cross, T.A.; Wang, J.; Tin, C.; Kim, S.; Kovacs, F.; Mo, Y.; Nishimura, K.; Gan, Z.; Fu, R.; Song, Z.; Denny, J. and Quine, J.R., Imaging membrane Protein Helical Wheels, 41nd Experimental NMR Conference, Asilomar, CA, April (2000)
  • Cross,T.A., NMR Spectroscopy at High Fields: An NMR Collaboratorium, 2nd Annual EMSL Workshop on Structural Genomics, Pacific Northwest National Laboratory, August (2000)
  • Cross,T.A., Wnag,J., Tin,C., Kim,S., Kovacs,F., Mo,Y., Nishimura,K., Gan,Z., Fu,R., Song,Z., Denny,J. & Quine, J., Imaging membrane Protein Helical Wheels, International Conference on Magnetic Resonance in Biological Systems, Florence, Italy, August (2000)
  • Cross,T.A.; Kim, S.; Wang, J. and Quine, J.R., Solid-State NMR: A Tool for High Resolution Macromolecular Structure Determination in a Bilayer Environment, Conference on the Frontiers in Structural Biology of Membrane Proteins, Galveston, TX, November (2000)
  • Cross,T.A.; Kim,S.; Wang, J. and Quine, J.R., Helical Wheels Directly Observed in Membrane Proteins by Solid State NMR, Pacifichem '00 International Chemical Congress Session Organizer, Honolulu, Hawaii, December (2000)
  • Denny, J.K.; Wang, J.; Quine, J.R. and Cross, T.A., Simulating Membrane Protein Helical Wheels from PISEMA Spectra, Annual Biophysical Society Meeting, New Orleans, LA, February (2000)
  • Grage, S.; Wang, J.; Mo,Y.; Cross, T.A. and Ulrich, A., Solid-state F-19 NMR Studies of Gramicidin A in Oriented Membranes, Experimental NMR Conference, Asilomar, CA, March (2000)
  • Kim,S.,Quine,J.R. & Cross,T.A., Complete Cross-Validation and R-Factor Calculation of Solid-State NMR Derived Structures, Experimental NMR Conference, Asilomar, CA, March (2000)
  • Nishimura, K.; Fu, R., and Cross, T., Heteronuclear Dipolar Recoupling in Solid-State MAS NMR in the Presence of RF Pulse Inhomogeneity: Practical Performance of REDOR and SFAM (poster), 41nd Experimental NMR Conference, Asilomar, CA, April 9-14 (2000)
  • Nishimura,J.,Fu,R. & Cross,T.A., Heteronuclear Dipolar Recoupling in Solid-State MAS NMR in the Presence of RF Pulse Inhomogeneity: Practical Performance of REDOR and SFAM, Annual Biophysical Society Meeting, New Orleans, LA, February (2000)
  • Quine, J. R. and Cross, T.A., Protein Structures from Solid State NMR: Mathematical and Technical Challenges, CIME Workshop on Protein Structure, Martina Franca, Italy, July 15 (2000)
  • Tian, C.; Wang, J.; Tobbler, J.; Lamb, R. and Cross,T.A., Structural Characterization by Solid-State NMR of Intact M2 Protein and the Transmembrane Peptide, Experimental NMR Conference, Asilomar, CA, March (2000)
  • Wang, J.; Denny, J.K.; Kim, S.; Mo, Y.; Nishimura, J.; Quine, J.R. and Cross, T.A., High Resolution Structure of the M2 Transmembrane Peptide (TMP) H+ Channel from Solid State NMR, Annual Biophysical Society Meeting, New Orleans, LA, February (2000)
  • Wang, J.; Nishimura, J.; Kim, S.; Kovacs, F.; Song, Z.; Mo, Y.; Quincy, J.R. and Cross, T.A., Structural Characterization of the M2 Transmembrane Peptide (TMP), Annual Biophysical Society Meeting, New Orleans, LA, February (2000)
  • Zhou, Z.; Macosko, J.C.; Hughes, D.W.; Sayer, B.G.; Hawes, J.; Shin,Y.K.; Epand, R.M.; Nishimura, J. and Cross,T.A., 15N NMR Study of the Ionization Properties of the Influenza Virus Fusion Peptide in Zwitterionic Phospholipid Dispersions, Annual Biophysical Society Meeting, New Orleans, LA, February (2000)